NphR
>
ATGGCTGAGACATTGCCACCGGTAACGGAATCGGCTGTTGCGCTACAGGCGGAGGTGACC
CAGAGGGAGCTGTTCGAGTTCGTTCTCAACGACCCCCTCCTCGCCAGTTCGCTGTATATT
AATATCGCACTGGCAGGGCTGTCGATACTGCTTTTCGTGTTCATGACGCGCGGACTCGAC
GACCCACGGGCGAAACTCATCGCCGTTTCGACGATTTTGGTGCCGGTGGTCTCTATCGCG
AGCTACACCGGCCTTGCATCGGGGCTCACCATCAGCGTCCTCGAGATGCCAGCCGGCCAC
TTCGCCGAGGGGTCCTCGGTGATGCTCGGCGGCGAAGAGGTAGACGGCGTCGTGACGATG
TGGGGCCGCTATCTGACGTGGGCCCTTTCGACACCGATGATACTGCTGGCGCTTGGGCTG
CTTGCTGGCTCTAACGCCACGAAGCTCTTTACCGCCATCACCTTCGACATCGCGATGTGT
GTCACCGGCCTCGCAGCCGCGCTGACGACCTCTTCGCACCTGATGCGGTGGTTCTGGTAC
GCCATCAGTTGTGCGTGTTTCCTCGTCGTCCTCTACATCCTGCTCGTCGAGTGGGCACAG
GACGCCAAGGCTGCCGGTACTGCGGATATGTTCAATACGCTGAAGCTGCTGACCGTTGTC
ATGTGGCTCGGCTACCCCATCGTGTGGGCACTCGGCGCTGAGGGCATCGCCGTTCTTCCG
GTCGGAGTCACGTCGTGGGGATACAGCTTCCTCGACATCGTCGCGAAGTACATCTTCGCG
TTCCTGCTGCTCAACTACCTCACGTCGAACGAGAGCGTCGTCTCCGGCTCGATACTCGAC
GTGCCGTCCGCGTCGGGCACTCCCGCTGACGACGGATCCCACCACCACCACCACCACTAA
Chr2 GFP
>
atggactatggcggcgctttgtctgccgtcggacgcgaacttttgttcgttactaatcct
gtggtggtgaacgggtccgtcctggtccctgaggatcaatgttactgtgccggatggatt
gaatctcgcggcacgaacggcgctcagaccgcgtcaaatgtcctgcagtggcttgcagca
ggattcagcattttgctgctgatgttctatgcctaccaaacctggaaatctacatgcggc
tgggaggagatctatgtgtgcgccattgaaatggttaaggtgattctcgagttctttttt
gagtttaagaatccctctatgctctaccttgccacaggacaccgggtgcagtggctgcgc
tatgcagagtggctgctcacttgtcctgtcatccttatccacctgagcaacctcaccggc
ctgagcaacgactacagcaggagaaccatgggactccttgtctcagacatcgggactatc
gtgtggggggctaccagcgccatggcaaccggctatgttaaagtcatcttcttttgtctt
ggattgtgctatggcgcgaacacattttttcacgccgccaaagcatatatcgagggttat
catactgtgccaaagggtcggtgccgccaggtcgtgaccggcatggcatggctgtttttc
gtgagctggggtatgttcccaattctcttcattttggggcccgaaggttttggcgtcctg
agcgtctatggctccaccgtaggtcacacgattattgatctgatgagtaaaaattgttgg
gggttgttgggacactacctgcgcgtcctgatccacgagcacatattgattcacggagat
atccgcaaaaccaccaaactgaacatcggcggaacggagatcgaggtcgagactctcgtc
gaagacgaagccgaggccggagccgtgccagcggcaccggtagtagcagtgagcaagggc
gaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggc
cacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctg
aagttcatttgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctg
acctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttc
aagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgag
ctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaac
tacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaac
ttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcag
aacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccag
tccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtg
accgccgccgggatcactctcggcatggacgagctgtacaagtaa
>
MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGTNGAQTASNVLQWLAA
GFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKNPSMLYLATGHRVQWLR
YAEWLLTCPVILIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWGATSAMATGYVKVIFFCL
GLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVSWGMFPILFILGPEGFGVL
SVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDIRKTTKLNIGGTEIEVETLV
EDEAEAGAVPAAPVVAVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
TAAGITLGMDELYK
pRT793 TEV pro
TEV protease, S219V mutant
>
CCGACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGA
GTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCG
GTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAA
CGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCAC
AACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGC
ACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCG
TGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATC
TTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCA
TTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGA
CACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATC
TGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGG
CGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAG
CGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGA
ATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAA
TGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACG
ACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTC
GCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGA
AGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATA
CGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTT
CCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAG
GCACAATTCTCATGTTTGACAGCTTATCATCGACTGCACGGTGCACCAATGCTTCTGGCG
TCAGGCAGCCATCGGAAGCTGTGGTATGGCTGTGCAGGTCGTAAATCACTGCATAATTCG
TGTCGCTCAAGGCGCACTCCCGTTCTGGATAATGTTTTTTGCGCCGACATCATAACGGTT
CTGGCAAATATTCTGAAATGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGA
ATTGTGAGCGGATAACAATTTCACACAGGAAACAGCCAGTCCGTTTAGGTGTTTTCACGA
GCACTTCACCAACAAGGACCATAGATTATGAAAATCGAAGAAGGTAAACTGGTAATCTGG
ATTAACGGCGATAAAGGCTATAACGGTCTCGCTGAAGTCGGTAAGAAATTCGAGAAAGAT
ACCGGAATTAAAGTCACCGTTGAGCATCCGGATAAACTGGAAGAGAAATTCCCACAGGTT
GCGGCAACTGGCGATGGCCCTGACATTATCTTCTGGGCACACGACCGCTTTGGTGGCTAC
GCTCAATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGACAAGCTGTAT
CCGTTTACCTGGGATGCCGTACGTTACAACGGCAAGCTGATTGCTTACCCGATCGCTGTT
GAAGCGTTATCGCTGATTTATAACAAAGATCTGCTGCCGAACCCGCCAAAAACCTGGGAA
GAGATCCCGGCGCTGGATAAAGAACTGAAAGCGAAAGGTAAGAGCGCGCTGATGTTCAAC
CTGCAAGAACCGTACTTCACCTGGCCGCTGATTGCTGCTGACGGGGGTTATGCGTTCAAG
TATGAAAACGGCAAGTACGACATTAAAGACGTGGGCGTGGATAACGCTGGCGCGAAAGCG
GGTCTGACCTTCCTGGTTGACCTGATTAAAAACAAACACATGAATGCAGACACCGATTAC
TCCATCGCAGAAGCTGCCTTTAATAAAGGCGAAACAGCGATGACCATCAACGGCCCGTGG
GCATGGTCCAACATCGACACCAGCAAAGTGAATTATGGTGTAACGGTACTGCCGACCTTC
AAGGGTCAACCATCCAAACCGTTCGTTGGCGTGCTGAGCGCAGGTATTAACGCCGCCAGT
CCGAACAAAGAGCTGGCAAAAGAGTTCCTCGAAAACTATCTGCTGACTGATGAAGGTCTG
GAAGCGGTTAATAAAGACAAACCGCTGGGTGCCGTAGCGCTGAAGTCTTACGAGGAAGAG
TTGGCGAAAGATCCACGTATTGCCGCCACCATGGAAAACGCCCAGAAAGGTGAAATCATG
CCGAACATCCCGCAGATGTCCGCTTTCTGGTATGCCGTGCGTACTGCGGTGATCAACGCC
GCCAGCGGTCGTCAGACTGTCGATGAAGCCCTGAAAGACGCGCAGACTAATTCGAGCTCG
AACAACAACAACAATAACAATAACAACAACCTCGGGATCGAGGGAAGGGGAGAAAATCTT
TATTTTCAAggtcatcatcatcatcatcatcatggagaaagcttgtttaaggggccgcgt
gattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacaca
acatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttaga
agaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaacacc
acgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaag
gatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcata
tgtcttgtgacaaccaacttccaaactaagagcatgtctagcatggtgtcagacactagt
tgcacattcccttcatctgatggcatattctggaagcattggattcaaaccaaggatggg
cagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagca
tcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattg
ttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaatgctgactcagta
ttgtgggggggccataaagttttcatggtgaaacctgaagagccttttcagccagttaag
gaagcgactcaactcatgaatcgtcgtcgccgtcgctaaTAAGGATCCTCTAGAGTCGAC
CTGCAGGCAAGCTTGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC
GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAA
GAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCAGCTT
GGCTGTTTTGGCGGATGAGATAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGA
AGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCC
ATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCG
AGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTT
TCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGC
GGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAAC
TGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACA
AACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTCTCCAATGATG
AGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
CTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTACCCCGGTTGATAATCAGA
AAAGCCCCAAAAACAGGAAGATTGTATAAGCAAATATTTAAATTGTAAACGTTAATATTT
TGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAA
TCGGCAAAATCCCTTATAAATCAAAAGAATAGCCCGAGATAGGGTTGAGTGTTGTTCCAG
TTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCG
TCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCAAATCAAGTTTTTTGGGGTCGA
GGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGG
GAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGG
CGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGC
CGCTACAGGGCGCGTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAA
ATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGA
TCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG
CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACT
GGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCAC
CACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTG
GCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCG
GATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGA
ACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCC
GAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACG
AGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTC
TGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCC
AGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTT
CCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACC
GCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGC
CTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACT
CTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTAC
GTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGG
CTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGT
GTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAG
CGTGGTCGTGCAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTT
TCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTT
CCTGTTTGGTCACTTGATGCCTCCGTGTAAGGGGGAATTTCTGTTCATGGGGGTAATGAT
ACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTT
ACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAAT
CACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCA
GCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTC
CAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGT
TTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGT
AAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCG
TGGCCAGGACCCAACGCTGCCCGAAATT
TagRFP
>
atgagcgagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaac
aaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagacc
atgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctacc
agcttcatgtacggcagcagaaccttcatcaaccacacccagggcatccccgacttcttt
aagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaagacgggggc
gtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaag
atcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgg
gaggccaacaccgagatgctgtaccccgctgacggcggcctggaaggcagaagcgacatg
gccctgaagctcgtgggcgggggccacctgatctgcaacttcaagaccacatacagatcc
aagaaacccgctaagaacctcaagatgcccggcgtctactatgtggaccacagactggaa
agaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccaga
tactgcgacctccctagcaaactggggcacaagtga
>
mselikenmhmklymegtvnnhhfkctsegegkpyegtqtmrikvveggplpfafdilat
sfmygsrtfinhtqgipdffkqsfpegftwervttyedggvltatqdtslqdgcliynvk
irgvnfpsngpvmqkktlgweantemlypadgglegrsdmalklvggghlicnfkttyrs
kkpaknlkmpgvyyvdhrlerikeadketyveqhevavarycdlpsklghk
Short sequences
NLS
ATGACTGCTCCAAAGAAGAAGCGTAAG
HA tag
TATCCTTATGACGTGCCTGACTATGCCAGCCTGGGAGGACCT
>YPYDVPDYASLGGP
5′ TAC CCA TAC GAT GTT CCA GAT TAC GCT3′
>YPYDVPDYA
Myc tag
GAGGAGCAGAAGCTGATCTCAGAGGAGGACCTG
>EEQKLISEEDL
HIS tag
CACCACCACCACCACCAC
>HHHHHH
Primers
Primer name Sequence 5′-3′
1492r tacggytaccttgttacgactt
16S-1392r acgggcggtgtgtgtrc
16S-27f agagtttgatcmtggctcag
35S-A AAGGGTCTTGCGAAGGATAG
35S-B AGTGGAAAAGGAAGGTGGCT
3′-Gal 4 GAACTTGCGGGGTTTTTC
BAC1 GCTCGTATGTTGTGTGGAATTG
BAC2 CCCAGTCACGACGTTGTAAAAC
BGH-Reverse TAGAAGGCACAGTCGAGG
CMV-F CGCAAATGGGCGGTAGGCGTG
EBV-RP GTGGTTTGTCCAAACTCATC
EGF1 CCAAGCTATTTAGGTGACAC
EGF2 GGGGATGTGCTGCAAGG
GFPN1-5 GAGCTCAAGCTTCGAATTC
GLprimer1 TGTATCTTATGGTACTGTAACTG
KAN2-FP ACCTACAACAAAGCTCTCATCAACC
KAN2-RP GCAATGTAACATCAGAGATTTTGAG
KS TCGAGGTCGACGGTATC
LPP1 atacgactcactatagggagac
LPP2 acaaattggactaatcgatggc
M13-FP TGTAAAACGACGGCCAGT
M13-RP CAGGAAACAGCTATGACC
pACT2-FP GATGATGAAGATACCCCAC
pACT2-RP CAGTTGAAGTGAACTTGC
pBacPAC-RP GTCTGTAAATCAACAACGC
pBAD-FP ATGCCATAGCATTTTTATCC
pBakPAC-FP TAAAATGATAACCATCTCGC
pBR1 CGAAAAGTGCCACCTGAC
pBR2 GGAGCCACTATCGACTAC
pBR3 TCCCCATCGGTGATGTC
PBR4 CTCGGCACCGTCACC
pc3.1GFP-Topo-RP CCCATTAACATCACC
pcDNA1.1-RP CTCTGTAGGTAGTTTGTCC
pcDNA1.1-RP/1 TAGAATCAGTAGTTTAACAC
pcDNA3.1-FP ctctggctaactagagaac
pcDNA3.1-RP/1 CAAACAACAGATGGCTGGC
PCR1 CGGGCCTCTTCGCTATT
PCR1minus1 GGGCCTCTTCGCTATT
PCR2 TTAGCTCACTCATTAGG
pDONOR-FP TAACGCTAGCATGGATCTC
pDONOR-RP GCAATGTAACATCAGAGAT
pEGFP_C2-FP GATCACATGGTCCTGCTG
pEGFP_C2-RP TTTAAAGCAAGTAAAACCTC
pEGFP_N CCGTCCAGCTCGACCAG
pEGFP-FP TTTAGTGAACCGTCAGATC
pEGFP-RP AACAGCTCCTCGCCCTTG
pESP-RP TCCAAAAGAAGTCGAGTGG
pET-24a GGGTTATGCTAGTTATTGCTCAG
pET30er-FP tcatcattcttctggtctg
pET36b-FP GCTAAATTCGAACGCCAG
pETBlue-RP aatagctttaatgcggtag
pET-RP CTAGTTATTGCTCAGCGG
pGBT9-FP AGTGCGACATCATCATCG
pGBT9-RP CGTTTTAAAACCTAAGAGTCAC
pGEX-3 GGAGCTGCATGTGTCAGAG
pGEX3-RP TCAAGAATTATACACTCCG
pGEX-5 CTGGCAAGCCACGTTTGG
pGEX5-FP AACGTATTGAAGCTATCCC
pGFP-FP GAGTTTGTAACAGCTGCTG
pGL2 TCTTTATGTTTTTGGCGTC
pGL3-1 GAGCTGACTGGGTTGAAG
pGL3-RV3 CTAGCAAAATAGGCTGTCC
pIRES-RP TATAGACAAACGCACACCG
pJet1-FP actactcgatgagttttcgg
pJet1-RP tgaggtggttagcatagttc
pMalE TCAGACTGTCGATGAAGC
pMT-FP CATCTCAGTGCAACTAAAG
PolyA-B AAAAAAAAAAAAAAAAAAAAAAAB
PolyC-D CCCCCCCCCCCCD
PolyT-V TTTTTTTTTTTTTTTTTTTTTv
pQE-FP CGGATAACAATTTCACACAG
pQE-RP GTTCTGAGGTCATTACTGG
pQE-RP-50 CTAGCTTGGATTCTCACC
pREP-FP GCTCGATACAATAAACGCC
pRH-FP ctgtctctatactcccctatag
pRH-RP caaaattcaatagttactatcgc
pRSET-RP ATGCTAGTTATTGCTCAGC
pRSET-RPnew GGGTTATGCTAGTTATTGC
pTal-FP CGGGAGGTACTTGGAGCG
pTeSp-1 CCTCCATAGAAGACACC
pTeSp-2 GCCCTGCCACTCATCG
pTI2-1-1 GGCAAATATTCTGAAATGAGC
pTI2-1-2 GCGTTTCACTTCTGAGTTCG
pTrcHis-FP GACCGGAATTATCGATTAAC
pTrcHis-RP CTGATTTAATCTGTATCAGG
pTYB-FP GGAAGACGATTATTATGGG
pUC-F GCCAGTGAATTCGAGCTCGG
pUC-R TGCCTGCAGGTCGACTCTAG
pVL1 GGGTTTAACATTACGGATTTC
pVL2 GACGCACAAACTAATATCAC
pVP22-FP CGCTTCTCGCCCCAGAC
RPC ACAGCTATGACCATGATTACG
SK CGCTCTAGAACTAGTGGATC
SP6 ATTTAGGTGACACTATAGAA
T3 ATTAACCCTCACTAAAGGGA
T7 TAATACGACTCACTATAGGG
T7-981079 TAATACGACTCACTATAG
T7minus1 AATACGACTCACTATAGGG
TET-1 FP-1 gggtgcgcatgatcctctagagt
TET-1 RP-1 taaattgcactgaaatctagaaata
Topo-1 TCGGATCCACTAGTAACG
Topo-2 GTGTGATGGATATCTGC
Topo-2N AGATGCATGCTCGAGCGG
TriplEx5`LD GAAGCGCGCCATTGTGTTGG
UP-2 CGTTGTAAAACGACGGCC
UP-40 GTTTTCCCAGTCACGAC
pRS315
>
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCA
CAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTG
TTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGC
ACCATATCGACTACGTCGTAAGGCCGTTTCTGACAGAGTAAAATTCTTGAGGGAACTTTC
ACCATTATGGGAAATGGTTCAAGAAGGTATTGACTTAAACTCCATCAAATGGTCAGGTCA
TTGAGTGTTTTTTATTTGTTGTATTTTTTTTTTTTTAGAGAAAATCCTCCAATATCAAAT
TAGGAATCGTAGTTTCATGATTTTCTGTTACACCTAACTTTTTGTGTGGTGCCCTCCTCC
TTGTCAATATTAATGTTAAAGTGCAATTCTTTTTCCTTATCACGTTGAGCCATTAGTATC
AATTTGCTTACCTGTATTCCTTTACTATCCTCCTTTTTCTCCTTCTTGATAAATGTATGT
AGATTGCGTATATAGTTTCGTCTACCCTATGAACATATTCCATTTTGTAATTTCGTGTCG
TTTCTATTATGAATTTCATTTATAAAGTTTATGTACAAATATCATAAAAAAAGAGAATCT
TTTTAAGCAAGGATTTTCTTAACTTCTTCGGCGACAGCATCACCGACTTCGGTGGTACTG
TTGGAACCACCTAAATCACCAGTTCTGATACCTGCATCCAAAACCTTTTTAACTGCATCT
TCAATGGCCTTACCTTCTTCAGGCAAGTTCAATGACAATTTCAACATCATTGCAGCAGAC
AAGATAGTGGCGATAGGGTCAACCTTATTCTTTGGCAAATCTGGAGCAGAACCGTGGCAT
GGTTCGTACAAACCAAATGCGGTGTTCTTGTCTGGCAAAGAGGCCAAGGACGCAGATGGC
AACAAACCCAAGGAACCTGGGATAACGGAGGCTTCATCGGAGATGATATCACCAAACATG
TTGCTGGTGATTATAATACCATTTAGGTGGGTTGGGTTCTTAACTAGGATCATGGCGGCA
GAATCAATCAATTGATGTTGAACCTTCAATGTAGGGAATTCGTTCTTGATGGTTTCCTCC
ACAGTTTTTCTCCATAATCTTGAAGAGGCCAAAACATTAGCTTTATCCAAGGACCAAATA
GGCAATGGTGGCTCATGTTGTAGGGCCATGAAAGCGGCCATTCTTGTGATTCTTTGCACT
TCTGGAACGGTGTATTGTTCACTATCCCAAGCGACACCATCACCATCGTCTTCCTTTCTC
TTACCAAAGTAAATACCTCCCACTAATTCTCTGACAACAACGAAGTCAGTACCTTTAGCA
AATTGTGGCTTGATTGGAGATAAGTCTAAAAGAGAGTCGGATGCAAAGTTACATGGTCTT
AAGTTGGCGTACAATTGAAGTTCTTTACGGATTTTTAGTAAACCTTGTTCAGGTCTAACA
CTACCGGTACCCCATTTAGGACCACCCACAGCACCTAACAAAACGGCATCAACCTTCTTG
GAGGCTTCCAGCGCCTCATCTGGAAGTGGGACACCTGTAGCATCGATAGCAGCACCACCA
ATTAAATGATTTTCGAAATCGAACTTGACATTGGAACGAACATCAGAAATAGCTTTAAGA
ACCTTAATGGCTTCGGCTGTGATTTCTTGACCAACGTGGTCACCTGGCAAAACGACGATC
TTCTTAGGGGCAGACATAGGGGCAGACATTAGAATGGTATATCCTTGAAATATATATATA
TATTGCTGAAATGTAAAAGGTAAGAAAAGTTAGAAAGTAAGACGATTGCTAACCACCTAT
TGGAAAAAACAATAGGTCCTTAAATAATATTGTCAACTTCAAGTATTGTGATGCAAGCAT
TTAGTCATGAACGCTTCTCTATTCTATATGAAAAGCCGGTTCCGGCCTCTCACCTTTCCT
TTTTCTCCCAATTTTTCAGTTGAAAAAGGTATATGCGTCAGGCGACCTCTGAAATTAACA
AAAAATTTCCAGTCATCGAATTTGATTCTGTGCGATAGCGCCCCTGTGTGTTCTCGTTAT
GTTGAGGAAAAAAATAATGGTTGCTAAGAGATTCGAACTCTTGCATCTTACGATACCTGA
GTATTCCCACAGTTAACTGCGGTCAAGATATTTCTTGAATCAGGCGCCTTAGACCGCTCG
GCCAAACAACCAATTACTTGTTGAGAAATAGAGTATAATTATCCTATAAATATAACGTTT
TTGAACACACATGAACAAGGAAGTACAGGACAATTGATTTTGAAGAGAATGTGGATTTTG
ATGTAATTGTTGGGATTCCATTTTTAATAAGGCAATAATATTAGGTATGTGGATATACTA
GAAGTTCTCCTCGAGGGTCGATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAA
ATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGT
TAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAA
GAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAG
AACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGT
GAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAAC
CCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAG
GAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTG
CGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCG
CCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGC
CAGCTGGCGAAGGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCC
CAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGGCGAA
TTGGAGCTCCACCGCGGTGGCGGCCGCTCTAGAACTAGTGGATCCCCCGGGCTGCAGGAA
TTCGATATCAAGCTTATCGATACCGTCGACCTCGAGGGGGGGCCCGGTACCCAGCTTTTG
TTCCCTTTAGTGAGGGTTAATTCCGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGT
GTGAAATTGTTATCCGCTCACAATTCCACACAACATAGGAGCCGGAAGCATAAAGTGTAA
AGCCTGGGGTGCCTAATGAGTGAGGTAACTCACATTAATTGCGTTGCGCTCACTGCCCGC
TTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAG
AGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGT
CGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGA
ATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCG
TAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCGGCCCCCCTGACGAGCATCACAA
AAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTT
CCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCT
GTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCT
CAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCC
CGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTT
ATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGC
TACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTAT
CTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAA
ACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAA
AAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGA
AAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCT
TTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGA
CAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC
CATAGTTGCCTGACTGCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGG
CCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAAT
AAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCAT
CCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCG
CAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTC
ATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGAAAAAA
AGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATC
ACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTT
TTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAG
TTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGT
GCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAG
ATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCAC
CAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGC
GACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCA
GGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGG
GGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGGGTCCTTTTCATCACGTGCTATAAA
AATAATTATAATTTAAATTTTTTAATATAAATATATAAATTAAAAATAGAAAGTAAAAAA
AGAAATTAAAGAAAAAATAGTTTTTGTTTTCCGAAGATGTAAAAGACTCTAGGGGGATCG
CCAACAAATACTACCTTTTATCTTGCTCTTCCTGCTCTCAGGTATTAATGCCGAATTGTT
TCATCTTGTCTGTGTAGAAGACCACACACGAAAATCCTGTGATTTTACATTTTACTTATC
GTTAATCGAATGTATATCTATTTAATCTGCTTTTCTTGTCTAATAAATATATATGTAAAG
TACGCTTTTTGTTGAAATTTTTTAAACCTTTGTTTATTTTTTTTTCTTCATTCCGTAACT
CTTCTACCTTCTTTATTTACTTTCTAAAATCCAAATACAAAACATAAAAATAAATAAACA
CAGAGTAAATTCCCAAATTATTCCATCATTAAAAGATACGAGGCGCGTGTAAGTTACAGG
CAAGCGATCCGTCCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTA
TCACGAGGCCCTTTCGTC
